Saturday, August 31, 2019

Jacques †Louis David Essay

We know that through out our Art History many artists have made themselves a name in this world. We have learned about the different periods of art history and the artist involved in those periods. Listening about all theses artists really touched me. There is so many to talk about, but I have pick one. Jacques Louis David was a great painter painting was considered his strength. He is one I really liked. Jacques Louis David was born on August 30 1748 and then he died December 29 1825. David was considered â€Å"the most celebrated French artist of his day and a principal exponent of the late 18th-century Neoclassical reaction against the Rococo style† (Britannia Encyclopedia). His father had died when he was nine and his mother left him. His uncles who were architects took care of him. He was not considered a good student. â€Å"He had a facial tumor that impeded his speech, and he was always preoccupied with drawing. He covered his notebooks with drawings† (Jacques-Louis David. org). He never paid attention to his studies just focusing on his art. He once said, ‘I was always hiding behind the instructor’s chair, drawing for the duration of the class’† (Jacques-Louis David. org). He wanted to be a painter but his uncles and mother wanted him to be an architect. He followed his heart and went to study with Francois Boucher, who was the famous artist of that time. The times were changing and so was the art. Boucher decided to send David to his good friend Joseph Marie Vien to study with him. David ended up attending the Royal Academy well he was there. David around 1770 to 1774 attempted to win a scholarship. He tried four times before he finally won. â€Å"David attempted to win the Prix de Rome, an art scholarship to the French Academy in Rome, four times between 1770 and 1774; once, he lost according to legend because he had not consulted Vien, one of the judges. Another time, he lost because a few other students had been competing for years, and Vien felt David’s education could wait for these other mediocre painters. In protest, he attempted to starve himself to death. Finally, in 1774, David won the Prix de Rome,† (Jacques-Louis David. org). David later on ended up in Italy. He was very inspired by the work. â€Å"David filled twelve sketchbooks with material that he would derive from for the rest of his life,† (Jacques-Louis David. org). In 1780, David completed the work Belisarius Asking Alms, which he also exhibited in Paris. He used a very unique approach to this piece. â€Å"In which he combined a nobly sentimental approach to antiquity with a pictorial technique reminiscent of Poussin,† (Britannia Encyclopedia). This piece you can tell is what started it all. You can see that David was very into the classical period. This is a very good piece for an artist just starting his career. To me it looks realistic, but you can still tell it was idealistic. I really liked the use of color in this piece. The focus though does not just seem to be on one person, but one all three characters portrayed in the piece. It really fits in the period. I chose this piece because it was one of David’s first pieces that kick started his career into art. It was the very first piece he exhibited. Another piece that helped his career is Oath of the Horatii. He produced this in 1784. This was one of the pieces in the Neoclassicism period. This piece had to do with a social contract. â€Å"The artist references Enlightenment values while alluding to Rousseau’s social contract,† (Wikipedia). They are showing the oath as a act of being one, they are coming together. â€Å"The Oath between the characters can be read as an act of unification of men to the binding of the state,† (Wikipedia). It also shows gender issue clearly between the women and the men. The issue of gender roles also becomes apparent in this piece, as the women in Horatii greatly contrast the group of brothers. David depicts the father with his back to the women, shutting them out of the oath making ritual; they also appear to be smaller in scale than the male figures,† (Wikipedia). This piece was really good. It really caught my eye when we studied it in class. I chose this piece because I wanted represent a piece of David’s from the Neoclassicism period. This was one of his best. It marks the middle of his time. One last piece of David’s is Mars Being Disarmed by Venus and the Three Graces. This was the very last piece of work he created before he died. It was known to be his â€Å"great last work†. He worked on this from 1822 to 1824. In 1823 he had said, â€Å"This is the last picture I want to paint, but I want to surpass myself in it. I will put the date of my seventy-five years on it and afterwards I will never again pick up my brush,† (Wikipedia). He exhibited this in Brussels, where about 10, 000 people came to view this piece. I chose this piece because I wanted show the ending of this story. This story is the story of David. He had actually stopped on his own will. This is the piece that represented he ended with a bang. He had been painting for seventy-five years now. I really liked how he showed the end of a battle. It symbolizes the end of his career. There are many great artists in our world, many from a long time ago. There has been so much artistic work that has impacted us in many ways. We can only rely on artwork to tell us about these artists that no longer exist. Jacques Louis David was a very famous painter from the many periods. He had seventy-five years of work. His impact has still remained. Artists will still remain.

Friday, August 30, 2019

LLM Personal Statement

Dear Sir: Early in life, I knew that I would become a successful lawyer. When I was a child, I had a picture of myself reading â€Å"The Little Book of Lawyers†. The book is a simple introduction to law that was written to make it easy for a young reader to understand. At an early age, I always wanted to know how to defend myself and my family from injustice. My passion for the study of law grew when the landlord of the home where I lived with my parents, served us an eviction order. As a result of our problem, I started studying Lease Law when I was in high school to determine if our lawyer was pursuing our case correctly. Fortunately, we won the case and this inspired me to begin my law study at the Università   Statale di Milano. After completing my law studies, I commenced my training experience in the law firm of the lawyer who assisted my family in the eviction procedure. I have regarded him as a meticulous mentor who has provided me with vast knowledge, exposure and experience and trained me to work in an orderly, precise and professional manner. After almost two years of law practice, I have confirmed a strong desire to specialize in International Law.   My love for International Law started when I was at the University where I studied under the tutelage of great professors such as Fausto Pocar and Tullio Treves. To have a better grasp of International Law, I decided to work at Clifford Chance LLP, a firm known to have a very strong international network. It has been almost four years now since I began my law practice at Clifford Chance (CC).   Since Clifford Chance had offices in various countries, I gained a lot of experience while working with colleagues in other CC international offices. I am usually assigned to work with colleagues from the London headquarters and the New York offices but I am often given the opportunity to work with other offices, e.g. Germany and Spain. As I deal with various cases, I learn to understand the importance of International Law.   I was astonished by the incredible ways various jurisdictions intertwined together and how the rules of every country combined in a unique entity in order to allow parties to have business relationships all around the world. Nowadays, the world is a very small place due to globalization and the advancement of technology. Even the smallest business has to deal with customers from different countries in order to be competitive. In my practice, I have encountered questions, e.g. what happens when an agreement is ruled by a law different from the country where the party in default lives? How can I enforce a judgment issued in Italy if the debtor is in Portugal? What happens when a company in Russia has to deal with a debtor who was declared bankrupt in Italy? The above are few of the problems that a good international lawyer has to deal with.   These are not easy issues and a lawyer has to study and work a lot and have good teachers in order to know how to handle them correctly. At Clifford Chance, I had the opportunity to work on very big and important issues. We dealt with the bankruptcy of one of the main companies in our country. This company has subsidiaries all over the world, has business relationship with different countries, and has issued bonds on the market. Although the international issues were complicated, dealing with the problem was extremely interesting as I worked together with highly qualified professionals who helped me understand the complexities of every issue. My passion to specialize in International Law has strengthened my desire to further my studies and pursue LLM in International Business and Trade Law.   I have always been focused in the pursuit of my career and my studies and work performance will attest that I have been a meticulous scholar, and a result driven lawyer.   As I always believe that we should always strive for excellence, I tend to work in a precise manner so I can achieve the best result. This is something that I have learned in my work experience. I believe in the saying that if you want to be a good professional, you have to think that you must become the best professional. It is only in aiming high that you can reach the higher points of knowledge, professionalism and skills. I hope that you will grant my application to pursue my studies in (write the name of the university). I strongly believe that pursuing the LLM International Business and Trade Law program will allow me to grain a solid knowledge and exposure on issues related to international law that will lead me to become a skilled professional in this field of study. Thank you very much. Very truly yours, Aaron Ghirardelli

Thursday, August 29, 2019

Creative and Critical Thinking Questions Assignment

Creative and Critical Thinking Questions - Assignment Example Do the right and left hemispheres have interchanging roles? What are the functions of the four lobes? What is the best way to categorize visual memory and analysis? What are the differences of functions among the four lobes? Is there a functional relationship between thalamus and hypothalamus? What does the delusion of consciousness mean? When a person is intellectually analyzing a situation, which part of the brain is majorly utilized? How can a person understand that a part of the brain responsible for understanding that something is wrong with it? What happens in the cortex as it inhibits the sexual urges towards a mother? What is the best diagnosis to know a person suffering from Oedipus Complex of Freud? What did Ramachandran imply when he said that the brain can contemplate the vastness of interstellar space (Ramachandran 00:11)? How can a person make sense out of not being able to even recognize his or herself? What part of the brain’s lobes is responsible for interpret ation and auditory reception? What process does the brain take to separate auditory and visual senses?

Wednesday, August 28, 2019

Role of commercial banks in causing the financial crisis Term Paper

Role of commercial banks in causing the financial crisis - Term Paper Example On the other side of Europe, the G7 financial ministers have assumed the crisis to be an American problem until much of the European banking system effectively collapsed. In countries such as Germany, the bailing out of the major industries including the Hypo Real estate as well as European mega banks came to hit them hard as they are the big lenders. Other nations like Britain emulated what was happening and commendably made their banking systems to become national. By October 2008 many of the European countries including Canada had gone so far as to guarantee not only the deposits but as well the debts of the banks as well. The financial system in the world is the umbrella body that will be concerned with how all the institutions that deal with finances will work including the borrowing of money in the form of loans that will be repaid in a period that will be short or relatively longer. At times, the webs of debt and credit have always been fragile in times of panic, spreading problems from a part of the global economy to the other. The reason that is responsible for this is that when one link in the very intricate chain becomes weak and breaks and defaults on some debt, it can leave creditors hazardously short of funds, unable to assure the credit of other firms. In this way, the consequences of one failure can spread throughout the entire economy and hence the entire money market (Roubini and Mihm 117) . There are always marketing risks arising when financial institutions trade assets and liabilities as well as derivatives as opposed to holding them for longer investment, funding or hedging purposes, (Saunders and cornett 184). When this happens, the financial institutions are assumed to directly control the maturities of their assets and liabilities as well as the interest issues are concerned. As interest rates fall, many mortgage borrowers seek to repay their existing loans and refinance at a lower rate.

Tuesday, August 27, 2019

Workplace issues or disaster management Essay Example | Topics and Well Written Essays - 500 words

Workplace issues or disaster management - Essay Example d a theoretical framework discussion issues that related retention and bullying, as well as the reported organizational culture that was deemed contributory to exacerbation of the issue by being indifferent or not addressing these concerns. Steven (2002) confirmed that the workplace issue of intimidation or bullying has pervaded the nursing profession for a considerable length of time: â€Å"bullying behavior has occurred within the nursing profession across a variety of institutional settings, including nursing faculties and professional bodies, for at least seventy-five years† (Stevens, 2002, p. 190). The author was reported to be part of the investigation team. The nurses who were victims of intimidated reported cases of supervisors who â€Å"regularly yelled at the nurse in front of other staff and patients, withheld information she needed to perform her duties, and excluded her from meetings and social functions. Others were given the â€Å"silent treatment† (talk ing to them through third parties, talking about them as if they were not present). Continual criticism of a person’s work, personal appearance, and voice was another type of behavior reported by nurses in the survey† (Stevens, 2002, p. 190). Through the initiation of management, the intimidation and bullying were appropriately addressed, which eventually resulted in lower turnover. Financial Impact on the Organization: The workplace issues on intimidation or bullying have financial impact on the organization in terms of the high turnover rates where were evident. Due to the need to frequently recruit new nurses to replace those who resigned due to intimidation or bullying, the hospital spends more in terms of high acquisition and training costs. These costs take a toll in terms of minimizing the organization’s profits. Personnel Impact: There are significant impacts in terms of personnel through exhibiting lower morale, low job satisfaction, and low productivity as a result of intimidation or

Monday, August 26, 2019

Motives for European expansion in Africa in the 19th Century Essay

Motives for European expansion in Africa in the 19th Century - Essay Example The reliance of these nations on raw materials for industries damaged the balance of trade forcing the countries to seek other sources of raw materials and cheap labor. Competition from newly industrialized nations such as Germany forced other European countries to seek expansion into Africa. Europe started by establishing trading relations with African leaders and encouraged the rulers to trade exclusively with them and not any other nation. At first, the European traders had no interest expanding into the interior of Africa. The partnership was such that the African rulers had assured them of a constant supply of slaves from the interior. However, the rapid expansion of industries in Europe made the colonialists look for more supply of raw materials and cheap slave labor from Africa. The European industries and economy depended particularly on West Africa for development. The palm oil developed from Africa was in high demand as an industrial oil in Europe. There was greed among the Europeans for greater profits and economic growth. The greed meant intensifying and increasing industrial production that meant expanding deeper and deeper into Africa. European conquest of Africa was majorly driven by economic motives and the desire to increase economic growth back in Europe. The Europeans realized that by conquering Africa, they could secure a very cheap supply of raw materials hence success and overall economic prosperity at home. The economic conditions in Europe in the 19th century fueled the scramble for Africa.

Sunday, August 25, 2019

Measuring Attitudes Towards Exercise Among Secondary School Students Essay

Measuring Attitudes Towards Exercise Among Secondary School Students - Essay Example The main strength of Likert’s method is its simplicity and reliability in producing results which are accurate and useful. Once the statements have been finalized, the test can be taken by students and administered by teachers who need to have no prior knowledge about the system or behind the scenes workings of the survey. Since test takers can be forced to take a position on a statement, it can also work to provide answers for situations where a decision must be made one way or another. At the same time, the test has been criticized for being too simplistic in nature and not applicable to situations where there is a possibility of having more than one dimension of attitude. Despite this, I believe that the survey would benefit the health campaign tremendously since we would be able to tell the attitude the students have towards exercise in general and the various statements in the survey in particular. The attitude of a person towards a particular statement helps in judging their expected behavior to a great extent. Therefore, knowing this attitude will allow us to gear the campaign in a manner more suited towards changing the attitude of the students (if required) or educating them by removing any negative thoughts they have towards exercise. Knowing the attitude is akin to a marketing study which is conducted by a business for advertisement purposes and if we have a campaign for the students without knowing what they think then at best we would be preaching to the converted and at worse, we would be alienating them.

Olympics as a Platform for Political Protests Essay

Olympics as a Platform for Political Protests - Essay Example Some people believe that by using the Olympics as a platform for political issues, they exert pressure upon oppressive governments and such governments may be forced to change their regime. It is a fact that 2008 Olympics to be held in China has sparked lots of debate. The above writer believes that these protests are actually prompting the Chinese government to review their policies towards Tibet. The latter country is fighting for independence and in order to gain international support, there is a need to choose the right movement when all eyes are on the host country. Such people argue that if they were to choose another time like after the Olympics, very few countries will be interested in the problems of China. Also using sporting events as a platform for political issues allows other countries to demonstrate their disdain about the actions of those oppressive regimes. There are also claims that host countries need to foster the image of the Olympics. This means that they need to portray the peace and unity that is synonymous with the event. If a host country has not been doing this, then it can be seen as a form of hypocrisy. The Chinese government has been criticized for its human rights abuses. There have reports about how the Chinese government prevents its citizens from discussing issues about their government. Furthermore, the Chinese government normally discourages most foreigners form discussing their problems claiming that it has nothing to do with them; they believe that they are the only ones with a right to solve their problems. In light of these arguments, it would be quite unfair for such a government to hold the Olympics and claim that they foster the spirit of unity that is synonymous with the torch. (Rice, 2008) Some people believe that protests should be conducted in any platforms a long as there is room to be heard. Furthermore, they believe that sometimes members of the host country may not have the political freedom to do so consequently, the international community needs top help them in this. For instance, there are numerous goods in the US government that come form the Chinese government. But workers in that location have minimal access to basic resources. Most of them are subjected to harsh working conditions and low pay. They cannot complain about this situation because it may bring problems. Consequently, the international community needs to help the Chinese workers by voicing their complaints. These complaints will only be noticed when the country stands to loose something. For instance, if the international countries boycotts the Olympics, then chances are the Chinese government will loose a lot. It may therefore be promoted to change their worker's conditions due to these chang es. Arguments against using the Olympics as a platform for political protests 'Sport is sport and politics is politics. There has never been anyone who has belied the fact that sport is the best way of uniting peoples.' (Brincat, 2008)

Saturday, August 24, 2019

Interview Analysis Research Paper Example | Topics and Well Written Essays - 1000 words

Interview Analysis - Research Paper Example The brand name played a vital role in purchasing the laptop. Apple is a household name not only in the US but also across the globe. Everyone recognizes that the products produced or manufactured by Apple are world class. Apple as a company is the best company that deals with computers, phones, and other electronic devices. Apart from these devices, Apple is well known for its operating system, Mac OS. Its line of laptops is respected for being the best in the market. In developed countries, virtually everyone is using a MacBook as his or her most trusted laptop. MacBook is a brand name on its own and when combined with Apple it becomes a force to reckon with in the world of laptops. The name indeed played a major role when choosing the type of a laptop to purchase. From the interviewee, it is apparent that the speed and graphics of this computer model were the main reason for buying the laptop. Therefore, my advice to the marketing manager will revolve around these two factors and mainly on the processor speed. For Apple to continue enjoying the leader role in the computer market, it has to continue producing these kinds of computers. If possible, the management should improve the current model in terms of speed since their competitors are doing their best to lure customers to buy their products. To keep up with a competitive and open market one has to make high-quality computers that are not only defined by their speed but also their graphics. Presently, Apple is performing extremely well in this sector.

Friday, August 23, 2019

Smoking ban laws in public places Essay Example | Topics and Well Written Essays - 500 words

Smoking ban laws in public places - Essay Example Thus, a ban on smoking would fail (Proctor). However, if smoke-free laws are enforced, premature deaths can be reduced because many people would refrain from the habit. Indeed, intensive smoking that is associated with severe health consequences can be avoided if strict smoking laws are enforced (ACS CAN 5). Smoke-free laws are also appropriate because they reduce the number of smokers in a country. This can happen in two ways. First, smokers will have limited places where they can smoke freely. Since comprehensive smoking laws prohibit smoking in public places, many smokers will be forced to quit the habit. As a result, there will be a decrease in the number of active smokers in within a country. Second, young people who would otherwise become smokers will not have the opportunity to do so. Normally, when young people start smoking, they do so in places where there are no family members. Public places provide an ideal environment for starting the smoking habit (Guilfoyle). Opponents of smoke-free laws argue that tough smoking rules would encourage smoking in hazardous environments, potentially endangering the lives of smokers (Proctor). However, with smoke-free laws, prospective smokers will not have anywhere to start the habit (Guilfoyle). In addition, smoke-free laws are appropriate because they decrease health costs. Health costs are incurred by smokers and nonsmokers. Smokers are at a higher risk of developing smoking-related illnesses. Smoking can also worsen other chronic conditions such as asthma. Nonsmokers can develop health complications such as chest pain, stroke, and asthma from secondhand smoking. They can also increase their chances of contracting lung cancer. Opponents of smoke-free laws argue that although there may be a decrease in health costs, there will still be severe economic consequences. For instance, several multi-billion dollar cigarette industries would close down

Thursday, August 22, 2019

Understand a Child and Young Persons Development Essay Example for Free

Understand a Child and Young Persons Development Essay Physical development 0-3 months New babies start swallowing and sucking reflexes begin. They grasp your finger if you put your finger in their palm. If babies are held up on their feet they usually make stepping movements. When babies hear a sudden sounds or a light is suddenly switched on they will startle by moving their arms outwards and clenching their fists. Towards the end of 3 months babies look less curled up and startles less as they get used to their new surroundings. 3-6 months- By now they have grown in height and have gained weight. Babies are likely to cry less and learn the difference between day and night and are able to sleep through the night. They will start to smile and the sound of their parent’s voice, and start to lift and turn their heads. 6-9 months- Babies are more alert and turn their heads to see what is happening, they showing they are enjoying themselves by smiling and making noises. They can now react out to grab a toy and pass it from one hand to another. They try to communicate by making noises back to you as if they are talking and answering your questions. They also try to grab the spoon when you are trying to feed them at this age. Babies sit up with a support of their high chair or a person or propped up with pillows. They start rolling on their back from their fronts. 9-12 months- Most babies by now are starting to crawl and finding their way around, even if it’s rolling or shuffling. They now sit out without any support and they want to explore so much more. They pick up objects and become more skilled at this. They feed themselves with their fingers. 1-2 years- At this age children can stand up and hold onto furniture, they can crawl very quickly and being skilled at using their hands. They get repeative with things they do such as taking hat off or pulling socks off, this also gets the adults attention. At 18months they begin to find their feet more and enjoy the freedom it gives them. They now can sit on toys and push themselves along with their legs for example- a small truck. 2-4 years- At 2 years old children move confidently and enjoy walking and being able to choose what they want to play with. They may start running and climbing at this age or it could take them till 3 years, depends on the child. They now enjoy pedaling a tricycle or pushing it along with their feet. Some children at this age can walk upstairs using alternative feet. 4-7 years- Children are now using their hands everyday for themselves enjoying activities such as coloring, painting, threading, pouring, controlling a mouse on the computer, and using scissors. They are also able to catch and throw a ball, kick and control a ball. Being to write and using more fine motor skills. 7- 12 years- At this age children find writing and reading a lot easier, handwriting becomes neater and drawings. Also cutting things out is more accurate. From 9 onwards they can now read, write , draw. They have greater coordination and speed when carrying out both fine and large movements, Such as running, football. 12-16 years- Physical changes start happening to the body to prepare for adulthood. Girl’s puberty starts around 11 years, whereas boys may not start until they are 13 or 14 years old. This can cause embarrassment and fell anxious. 16- 19 years- Body changes to prepare for adulthood. Boys may get beards and their voices may start to change. Girls around the age of 15 or 16 will have finished puberty, whereas the boys may have started age 13 or 14 and is likely to take about 3 years to complete. Intellectual and cognitive development 0-3 months- Babies will recognize the smell and sounds of parents voice, they may stop crying when this happens knowing that they are safe. They also try hard to focus on your face, but hold them 20-25 cm away from you as they may be able to see you. 3-6 months- They start to notice mobiles and other objects around them and watch people move around. 6-9 months- Babies explore objects with their mouth as well as their fingers. They will spend a lot of time sitting and playing. Around 8-9 months babies understand that objects and people still exist even when they are not in sight. This is where they make a fuss when their familiar career leaves the room. 9-12 months- They explore objects with their hands and mouth. They start to recognize the daily routine, e.g. know when its dinner time, or bath time they get excited when maybe you start running the bath or put them in their highchair ready for dinner. 1-2 years- Children at this age start to understand more of what is happening around them, they understand more of what is people said. They also have an enjoyment of pop-up toys and play with building bricks and do simple jigsaw puzzles. They recognize the daily routines such as becoming excited when they hear the bath water running as they know its bath time. They start to want some independence at this age, and are developing as individuals. 2-4 years- Children at this age play with small world e.g. farm animals, peppa pig and pretend in the role play area they act out everyday thing and copy what they may have seen at home. E.g. mum washing up or dad making the dinner. They become interested in mark making, painting and books. 4-7 years- They will start to plan their own play and have definite ideas of what they want to do and achieve, they are also becoming even more independent. Children at this age concentrate when activity is interesting to them and have the ability to count and do simple calculations. They also read books silently on their own. 7-12 years- Children at this age can read books silently on their own, they also organize their own play and games and can problem solve e.g. how to play a game. And they enjoy practical situations in which they have to work things out for themselves. 12-16 years- At this age children can problem solve and reason on their own, they also understand the more abstract concepts. They also may try to push the boundaries at home and question why they are in place. 16-19 years- Young people at this age have a higher level of skills such as computers, mobile phone and all the latest technology. And they explore their own identity and develop own personality and create their own space. Communication development 0-3 months- Babies start to cry when hungry tired or distressed, they also start to coo when contented for about 5 weeks. Babies cry to communicate their needs and parents may start to understand the different is types of cries. Babies may stop crying when they hear their parents soothing voice. 3-6 months- At this age babies will start to smile back when they see a smiling face they also start to show they understand a little of what is being said to them. They try to communicate back by cooing and gargling 6-9 months- Babies arms start lifting up to show their parents or carer they want to be picked up they show they understand a little of what is being communicated to them by smiling and squealing 9-12 months- Babies are developing language at this age, they start to make tuneful strings of babbling. They start to point fingers to draw attention of parents for objects they want and have an understanding of what some words mean e.g. get excited when they hear words such as drink or dinner. 1-2 years- At 1 year old strings of babbling are still they way in which babies try to communicate but are beginning to use first words which are usually noticed bye their parents or carers around the age of 13 months. Children at 18 months are still developing their language skills, they start to use a few words e.g. ‘ juice’ ‘muma’ ‘dada’ they understand a lot more of what adults are saying to them. Towards the age of two years they may have a vocabulary of up to 200 words 2-4 years- Children at this age start putting together two words such as ‘daddy gone’ and their speech begins to be understood by adults. By three years their language is really starting to develop they express their ideas and are even starting to use sentences. Children at this age start to pick up what is being said around them and may use this in their communication. 4-7 years- Speech is now easily understood they are beginning to develop friendships and enjo y communicating with their friends and asking them questions. At the age of 5 plus they have the ability to socialize such as beginning to hear and make jokes. 7-12 years- At this age they are telling a lot more jokes and enjoy chatting to their friends. They will start to have verbal arguments with friends and family they also can become persuasive and make deals with people. At 9 years old they can now read write and draw and show imagination. 12-16 years- At this age they have good reading and writing skills they also can be persuasive and make deals with adults and peers. They can use phones and other technologies to communicate such as instant messaging sites. 16-19 years- At this age the use of phones and technology is very important to them to communicate with their friends. Friends are very important at this age more important than being with their family because they feel they are better to go to for support and advice. Social, emotional and behavioral development 0-3 months- Babies love close contact between their primary carers , especially when their feeding. Babies smile when they are asleep and they begin to smile when content around 5 weeks old. 3-6months- They sleep a lot less at this age, and smile more often as they are alert of parent’s voice and movement. They enjoy bath time. 6-9months-Babies around 6 months are usually settled in a routine. They smile a lot when they are playing with familiar people. 9-12months- Babies try to stay close to their parents or carers at this age. They will protest when they leave the room as they know they are gone and it might work to get them to come back. 1-2years- Children at this age stay close to their parents or carers and can get anxiety if people they don’t know come over to them or pick them up. This is the age they become interested in playing with other children, and appear to have signs of temper and frustration. 2-4 years- Children at this age play next to other children but not necessary with them, they copy what other children do and put this into play. Temper tantrums can become more frequent at this age. By the age of 3 years some children may play with other children more and they become more cooperative in play. 4-7 years- At this age they start to play with other children and they begin to form friendships and know who they like to play with, and may seek this friend out to play with. They may have the odd argument with their friend over a toy but it is soon resolved. They also respond well to adults praise. 7-12 years- Children have stable friendships by now and are going over to friend’s houses for tea and maybe over night. They have enthusiasm when given responsibility. E.g. when asked to watch little brother or sister. 12-16 years- At this age they have very strong attachments with friends and this is very important to them. They may experience pressure at school and become anxious about going to school. They start to try and push boundaries in their relationships with their parents. 16-19years- Young adults have confidence and enjoyment when it comes to relationships with their peers. They may feel left out if they don’t have a group of friends. Behaviour could be linked to low self esteem which could lead to drinking, drugs and early promiscuity. Moral development 0-3months- Babies have their first smile at about 5 to 6 weeks old. 3-6 months- Babies show joy by smiling, cooing and laughing when they are fed, comfortable or safe. 6-9 months- At this age babies show joy by smiling, cooing and laughing when they are fed, comfortable or safe. They cry when distressed or unhappy. 9-12 months-Babies at this age have no understanding of right or wrong, but starts to understand word ‘NO’ when applied with a firm voice. 1 2 years Sensitive to adult approval and disapproval, despite tantrums and bursts of anger. No understanding of right from wrong, but understanding yes or no. 2-4 years –No understanding of right or wrong but start to follow simple rules most of time. By the age of 3 and a half they are beginning to know right from wrong and are more self-controlled and less aggressive. They want to be good, but is not yet sure how to carry out most promises. 4-7 years –Children at this age are willing to be good, but may tell lies or blame others for doing wrong because of intense desire to please and do right. 7-12 years –Children at this age may experience guilt and shame. They have difficulty admitting mistakes. Is aware of right and wrong and they want to do right. 12-16 years –Children by now know right and wrong, they try to weigh up alternatives and make some decisions alone .And are usually reasonably thoughtful and at this age they are unlikely to lie. 16-19 years –Young adults at this age experiences feelings of frustration, anger, sorrow, and isolation. May become confused and disappointed and this could result in being interested in early promiscuity as response to physical-emotional urges.

Wednesday, August 21, 2019

Biochemical Analysis of Rice

Biochemical Analysis of Rice Rice (Oryza sativa (2n = 24) is a monocot plant and belongs to the Poaceae family and Oryzoidea subfamily. It occupies almost one-fifth of the total land area under world cereals. It covers about 148 million hectares annually that is roughly 11 percent of the world-cultivated land. It is life for more than half of humanity and in past, it shaped the cultures, diets, and economies of billions of people in the world (Farooq et al., 2009). More than 90 percent of the worlds rice is grown and consumed in Asia where 60 percent of the world population lives. The world major rice consuming countries are China, India, Egypt, Indonesia, Malaysia, Bangladesh, Vietnam, Thailand, Myanmar, Philippines, Japan, Brazil, South Korea and USA that consume 135, 85, 39, 37, 26, 18, 10, 10, 9.7, 8.7, 8.1, 5.0 and 3.9 million metric ton, respectively (Meng et al., 2005; USDA, 2003-04). Biochemical and nutritional aspects of rice Rice is a major source of macro and micronutrients for human being. It feeds more than two billion people worldwide and is the number one staple food in Asia. It provides over 21 percent of the calorific needs of the worlds population and up to 76 percent of the calorific intake of the population of South East (SE) Asia (Fitzgerald et al., 2009). It is mostly consumed as a polished grain, which usually lacks its nutritional components such as minerals and vitamins 41 P. Lucca et al., Genetic engineering approaches to enrich rice with iron and vitamin A, Physiol. Plant. 126 (2006), pp. 291-303. Full Text via CrossRef | View Record in Scopus | Cited By in Scopus (7)( Lucca et al., 2006). Since the advent of molecular techniques, recently genetically modified rice verities have been developed, which contains more nutritional aspects like minerals and vitamins in endosperm (Vasconcelos et al., 2003; Paine et al., 2005; Fitzgerald et al., 2009). The major value-added nutritional protein c onstituents of the rice. Rice Position in Pakistan In Pakistan, besides its importance as a food crop, rice is the second important component of daily diet of bulk of the population after wheat. About 23% of the total foreign exchange earnings is shared by rice and thus called as Golden Grain of Pakistan (Shah et al., 1999). Around one third of total production is annually exported and two third is locally consumed to meet food needs. Rice is also used in dishes for special occasions (Sagar et al., 1988). Pakistan is the third largest rice exporting country. In Pakistan, rice occupies about 10% of the total cultivated area, accounts for 6.1% of value added in agriculture and 1.3% in gross domestic product. Production of rice during 2007-08 was estimated at 5,540 thousand tones, 10.4% higher than last year with 6.1% increase in yield per hectare (Anonymous, 2006). Area, production and yield of rice for the last 5 years are shown in Fig. 1. Varieties of basmati rice, sub-species of indica, are economically important due to the high quality of the grain and constitute an important source of revenue for two major rice-growing countries in Asia (Pakistan and India). The international market for basmati rice has always been higher than that of the moderate varieties. Pakistans annual rice export stands at about 2.5 million tons, which earn a total of 513.0 million dollars for the country (Anonymous, 1998). During the year 2005-2006 rice export was about one billion US$ (Bashir et al., 2007). Rice growing areas of Pakistan Depending upon the irrigation water availability, rice can be grown in any part of the country from sea level up to 2500m height. Pakistan has a climate and a potential in soil that permits the expectations of a most bright future for the productions of rice. Considering temperature difference, optimum sowing seasons and the varietals performance, rice growing areas can be divided in four ecological zones (Salim et al., 2003; Table-1.2). Rice is grown in all four provinces of Pakistan. However, the acreage under rice varies greatly from one province to another. The Punjab and Sindh are the major rice growing provinces with about 59% and 33%, respectively of the total rice in the country. The remaining 5% of the area is planted in Baulochistan and 3% in NWFP (Bhatti and Anwar, 1994). Despite the fact that its cultivated area is far smaller than wheat (more than 7.24 million), it has a great impact on national economy due to two reasons. Firstly, rice is the only crop which can be grown successfully in vast chunks of salt-ridden and water-logged areas where it facilitates not only the reclamation of land for the cultivation of other crops but also provide food. Secondly, superior quality basmati has a consistently increasing demand in the foreign countries. Consequently, there is a great scope for augmenting the foreign exchange earning by exporting it in bigger quantity. In view of these facts, it is highly desirable to increase the production and improve the quality of rice the quality is particularly more important from the trade view point, as it is instrument entail in increasing and then sustaining the demand in the foreign market in competition with other rising exporting countries. There in no denying the fact that purity is the very sole of quality. The impurities not only restrict the export trade, but also inflict losses to the growers, millers and the consumers alike. Therefore, these should possibly be minimized (Saleem et al., 2003). Major rice varieties in Pakistan More than 20 rice varieties have been released for general cultivation in Pakistan (Bashir et al., 2007). A general description of agronomical and physiochemical characteristics of these varieties. Importance of Basmati Rice in Pakistan There are thousands of rice varieties and landraces, which differ with respect to plant and grain characteristics. Of these, aromatic (Basmati) rice constitutes a small but special group that is regarded as best in grain quality, superior aroma and usually used for special dish preparation (Khush and dela Cruz, 2001). Quality of rice may be considered from the view point of size, shape and appearance of grain, milling quality and cooking properties (Dela Cruz and Khush, 2000). Pakistan is famous for the production and export of Basmati rice. The origin of the word Basmati can be trade to the word Basmati meaning earth recognized by its fragrance. The Hindi word Bas was derived from the Pakrit word BAS and has a Sanskrit root Vassy (Aroma), while Mati originated from Mayup (ingrained from the origin). In common usage Vas is pronounced as Bas and while combining Bas and Mayup, the later changed to Mati thus the word Basmati (Ahuja et al., 1995; Gupta, 1995). The fragrance of basmati rice is most closely associated with the presence of 2-acetyl-1-pyrroline (Buttery et al., 1983; Lorieux et al., 1996; Widjaja et al., 1996; Yoshihashi et al., 2002). Although many other compounds are also found in the headspace of fragrant rice varieties (Widjaja et al., 1996) possibly due to secondary effects related to the genetic background of the rice variety, 2-acetyl-1-pyrroline is widely known to be the main cause of the distinctive basmati and jasmine fragrance. The desirability of fragrance has resulted in strong human preference and selection for this trait. Non-fragrant rice varieties contain very low levels of 2-acetyl-1-pyrroline, while the levels in fragrant genotypes are much higher (Widjaja et al., 1996). Basmati rice occupies a prime position in the Indian subcontinent and is becoming increasingly popular in Middle East, Europe, USA and even in non-traditional rice growing countries such as Australia (Bhasin, 2000). High-quality, traditional Basmati rice varieties command premium prices, more than three times that of non-Bamati rices in the world market due to its exquisite aroma, superfine grain characteristics and excellent cooking (extra elongation, soft and flaky texture) qualities (Bhasin, 2000; Singh et al., 2000a; Khush and dela Cruz, 2002). Basmati rice traditionally grown in the Himalayan foothills regions of Pakistan and India, and the name is traditionally associated with this region. Basmati rice is the result of centuries of selection and cultivation by farmers (Khush, 2000). Cultivation of basmati rice in mainly confined to the Kallar tract (Gujranwala, Sheikhupura and Sialkot districts) of Punjab province. Basmati rice always fetch a higher price in the domestic as well as in the international market due to their peculiar quality features such as pleasant aroma, fine grain, extreme grain elongation (7.6mm long) and soft texture on cooking. In spite of hard competition from India, Thailand and the United States, Pakistan enjoys a good position in the global trade of aromatic rice and every year earns a lot of foreign exchange (Akram and Sagar, 1997). Genetic Diversity in Rice Diversity among organisms is a result of variations in DNA sequences and of environmental effects. The diversity in crop varieties is essential for agricultural development for increasing food production, poverty alleviation and promoting economic growth. The available diversity in the germplasm also serves as an insurance against unknown future needs and conditions, thereby contributing to the stability of farming systems at local, national and global levels (Singh et al, 2000). In crop improvement program, genetic variability for agronomic traits as well as quality traits in almost all the crops is important, since this component is transmitted to the next generation (Singh, 1996). Study of genetic divergence among the plant materials is a vital tool to the plant breeders for an efficient choice of parents for plant improvement. Genetically diverse parents are likely to contribute desirable segregants and/or to produce high heterotic crosses. Parents identified on the basis of dive rgence for any breeding program would be more promising (Arunachalam, 1981). In early 1970s, public authorities felt the need that genetic resources should be collected, maintained and conserved, especial focus was on important food crops e.g wheat, rice, barley etc (Hawkes 1983; Bellon et al., 1998; Barry et al., 2007). This was the first official attempt to preserve genetic diversity. Currently different genetic diversity assessment methods including morphological, biochemical and molecular markers are available. Morphological Markers used to study genetic diversity Morphological evaluation is the oldest and considered as the first hand tool for detection of genetic variation in germplasm (Smith and Smith, 1989). It is cheap and convenient. It requires not any in depth knowledge at genomic or proteomic level. However, morphological markers are relatively less effective for genetic diversity analysis due to sensitivity to environmental influences and developmental stage of the plant (Werlemark et al., 1999). It takes long time, requires seasonal changes and quite laborious. The genetic variability for some of the traits needed for high yield performance and stress tolerance is limited in cultivated germplasm. This is because a small core of adapted progenitors has been used repeatedly in rice breeding programs such that the genetic base of rice has become narrow (Moncada et al. 2001; Hargrove et al. 1980; Dilday 1990). Introgression of genes from other rice species can provide genetic variation to improve rice and meet the challenges affecting ri ce production. Morphological traits including both qualitative and quantitative ones are used to evaluate genetic relationship among genotypes (Goodman 1972; Bajracharya et al., 2006). Fida et al. (1995) reported the evaluation of elite rice genotypes for agronomic traits during 1992 at NARC, Islamabad. All the genotypes possessed similar grain quality. Agronomic evaluation was used for screening of lines with desired performance by Akram et al. (1995), in field leading to the identification of varieties possessing longer and fine grains as donors for utilization in breeding programmes aimed for the improvement of grain length in Basmati rice. Iqbal et al. (2001) morphologically evaluated selected landraces for paddy yield and other important agronomic traits as a propose to select parents for hybridization program. All the landraces possessed some desirable agronomic traits so these proved effective in rice breeding programmes. Koutroubas et al. (2004) described variation in grain quality traits among some European rice lines. They concluded that these lines could be used as parents for introgression of desired traits into different rice cultivars grown in Europe. They also suggested that the interrelations among grain quality traits found in these lines could be useful to study the relationship among their grain quality components and for improving selection criteria. Nabeela et al. (2004) evaluated fifteen agronomical important traits in landrace genotypes of rice collected from various parts of Pakistan. A significant amount of genetic variation was displayed for most of the traits examined. The coefficient of variation was more than 10% for all the characters with exception of grain length. Seven accessions with best performance for individual character were identified, by exploiting their genetic potential. These genotypes can have a beneficial use in the breeding programs. Nepali rice landrace diversity was evaluated by Bajracharya et al. (2005) by usin g morphological traits as one of the parameter for selection. The genotypes varied only for few quantitative traits controlled by major genes; husk color, seed coat and panicle traits. Agronomic characterization also helped to decide which traits need to be improved for further crop improvements. Zaman et al. (2005) studied fifteen different rice varieties which showed that the different morphological characteristics such as the yield, tiller number per hill and filled grains per panicle did not contribute towards the total divergence. This suggested that the breeding improvement of these morphological characteristics have the little possibility. Little phenotypic variation at farm level was observed in Vietnamese rice by Fukuoka et al. 2006, which was considered to be the result of genetic drift and selection by the farmers, on farm conservation of the landraces of rice is considered to be under a force to decrease phenotypic diversity. Different phenotypic profiles contribute to t he conservation of regional genetic diversity of the landraces of rice. Veasey and colleagues (2008) investigated the genetic variability among different rice species from South in a greenhouse experiment. They showed a significant difference (p Keeping in view these benefits, morphological variation is a selection criterion for plant scientists among landrace genotypes. Though the environmental factors also play an important role in morphological variation but the knowledge of agro-morphological diversity and the distribution pattern of variation among crop species could be an invaluable aid in germplasm management and crop improvement strategies. Zeng et al. (2003) studied ecogeographic and genetic diversity based on morphological characters of rice landraces (Oryza sativa L.) in Yunnan, China. A great difference in ecological diversity index of rice resources between prefectures or counties in Yunnan province exists. Kayode et al. (2008) studied the relationship in geographical pattern and morphological variation of 880 rice landrace in CÃ ´te d’Ivoire for 13 agro-morphological characters. Result of the phenotypic frequency showed differential distribution of landraces with height, heading and maturity period whic h reflected the distribution pattern of different Oryza sativa landraces in CÃ ´te d’Ivoire that proved useful in germplasm management and breeding programs. The altitudinal distributions of grain length, grain width, grain length to width ratio and grain weight were evaluated by Siddiqui and coworkers in 2007. It was noticed that grain length decreased with the increase in altitude, while the grain width increased with the increase in altitude, resulting into a decrease in length to width ratio with the increase in altitude. Considering the change in altitude as a difference in habitat and environment, it can be assumed that Pakistan rice cultivars show a wide variation between and within locations. It may be concluded that the Pakistan rice genetic resources comprise of great diversity for grain morphological characteristics. The prevailing diversity for grain type (shape and size) and pericarp color has distinct correlation to its geographical distribution in terms of alt itude. Morpho-physiological traits are an important tool in hands of plant breeders for identification and purity testing of rice varieties. Sharief et al. (2005) investigated the genetic purity of four different rice varieties on the basis of morphological characteristics at their different growth stages. All of the varieties were identified by different morphological characteristics in terms of flag leaf area, grain color, seed width, number of tillers, time of heading, absent awing, slemma, palea pubescence, plant height, and culm diameter. Biochemical markers for analysis of diversity Seed proteins are very helpful in genetic diversity evaluation in cereal crops because the seeds of these crops have nutritional value. Glutelin, globulin and prolamin are important seed proteins in rice. Variation in these proteins at subunit level changes the quality of rice. Various tools were used to assess variability at peptide level. Biochemical markers have some disadvantages being tissue specific and affected by environmental and developmental changes. These disadvantages could be eliminated by the use of seed storage protein as they are conservative in nature and least effected by environmental changes. (Thanh et al., 2006) Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE) is useful method not only for revealing variations but also for identification of a variety in seed storage proteins. Four protein fractions (albumin, globulin, gliadin and glutenin) separated by SDS-PAGE as biochemical marker for evaluating polymorphism in three spelt wheat varieties. Very significant difference was observed at protein profile level in old cultivars and new breeding lines (Dvoracek and Curn 2003). Sengupta and Chattopadhyay (2000) identified twelve rice varieties on the basis of banding pattern obtained by SDS-PAGE. Aung et al. (2003) investigated 350 local rice cultivars from different regions of Myanmar. These were analyzed by using SDS-PAGE and IEF. Various cultivars differed in their SDS-PAGE profiles. Padmavathi et al. (2002) evaluated seven aromatic and five non-aromatic rice cultivars using SDS-PAGE. Two bands of 60.3 and 51.3KDa were polymorphic for their presence in both aromatic and non-aromatic genotypes and suggested that these polymorphic bands can be used as markers for verification of hybridity in crossing programme. Rehana et al. (2004) investigated twenty accessions of Pakistani rice germplasm for total seed protein by using SDS-PAGE, to determine the magnitude of genetic variation with respect to geographical distribution. Vari ation in protein banding pattern with respect to various geographical regions was evaluated and it was suggested that the inter-specific variations were more pronounced as compared to intra-specific variations. Variation in banding profile of globulin and glutelin was used as identification tool for differentiating coarse, fine and super fine rice cultivars by Thind and Sogi (2005). Jahan et al. (2005) studied protein diversity in 576 rice cultivars from Bangladesh and SDS-PAGE was used for separation. Thanh et al., 2006 used seed storage protein profiles of different varieties including rice for evaluation of genetic purity and variability. Molecular markers for diversity analysis Variation in a DNA sequence is known as DNA polymorphism. This quality of DNA can be used as a marker to assess diversity in the genome of any organism. An ideal DNA marker must have any of the following qualities: Highly polymorphic in nature, co-dominant inheritance, frequent occurrence in genome, selective neutral behaviour, easy access/availability, easy and fast assay, high reproducibility and easy exchange of data between laboratories (Joshi et al., 1999). DNA-based molecular markers/DNA fingerprinting can increase screening efficiency in breeding programs in a number of other ways. For example, they provide: the ability to screen in the seedling stage for traits that are expressed late in the life of a plant (i.e. grain or fruit quality, male sterility, photoperiod sensitivity), the ability to screen for traits that are extremely difficult, expensive, or time consuming to score phenotypically (i.e. root morphology, resistance to quarantined pests or to specific races or biotyp es of diseases or insects, tolerance for certain abiotic stresses such as drought, salt, or mineral deficiencies or toxicities), the ability to distinguish the homozygous versus heterozygous condition of many loci in a single generation without the need for progeny testing (since molecular markers are co-dominant), and the ability to perform simultaneous marker-aided selection for several characters at one time. Random Amplified Polymorphic DNAs (RAPDs) Randomly-amplified polymorphic DNA markers (RAPD) are arbitrary sequence markers developed by Welsh and McClelland in 1991. This procedure detects nucleotide sequence polymorphisms in DNA by using a single primer of arbitrary nucleotide sequence. In this reaction, a single species of primer anneals to the genomic DNA at two different sites on complementary strands of DNA template. If these priming sites are within an amplifiable range of each other, a discrete DNA product is formed through thermocyclic amplification. On an average, each primer directs amplification of several discrete loci in the genome, making the assay useful for efficient screening of nucleotide sequence polymorphism between individuals. However, due to the stoichastic nature of DNA amplification with random sequence primers, it is important to optimize and maintain consistent reaction conditions for reproducible DNA amplification. They are dominant markers and hence have limitations in their use as markers for ma pping, which can be overcome to some extent by selecting those markers that are linked in coupling. RAPD assay has been used by several groups as efficient tools for identification of markers linked to agronomically important traits, which are introgressed during the development of near isogenic lines. though it is less popular due to problems such as poor reproducibility faint or fuzzy products, and difficulty in scoring bands, which lead to inappropriate inferences but it is still applied as markers in variability analysis and individual-specific genotyping has largely been carried out,. Raghunathachari et al. (2000) differentiated a set of 18 accessions from Indian scented rice by random amplified polymorphic DNA (RAPD) analysis. The RAPD analysis offered a rapid and reliable method for the estimation of variability between different accessions, which could be utilized by the breeders for further improvement of the scented rice genotypes. Porreca et al. (2001) reported confirmati on of genetic diversity among 28 rice cultivars, different for biometric traits, biological cycle and suitability to water limitation, using RAPD markers. High level of polymorphism was found between japonica and indica subspecies, whereas japonica cultivars with long grains (tropical) resulted to be genetically different from the short grains genotypes (temperate). Genetic relationships among indica and japonica cultivars and between tropical and temperate japonica was estimated. Variability among the varieties could lead to good heterotic combinations between japonica genotypes. Neeraja et al. (2002) determined genetic diversity in a set of landraces in comparison to a representative sample of improved rice varieties, using random amplified polymorphic DNA (RAPD). Analysis of 36 accessions using 10 arbitrary decamer random primers, revealed 97.16% polymorphism. Similarity values among the landraces ranged from 0.58 to 0.89 indicating wide diversity. The landraces and improved vari eties formed separate clusters at 0.65 similarities suggesting that genetically distant landraces could be potentially valuable sources for enlarging and enriching the gene pool of improved varieties. Kwon et al. (2002) evaluated genetic divergence among 13 Tongil type rice cultivars and the relationship between genetic distance and hybrid performance in all possible nonreciprocal crosses between them assessed. These results indicate that GDs based on the microsatellite and random amplified polymorphic DNA (RAPD) markers may not be useful for predicting heterotic combinations in Tongil type rice and support the idea that the level of correlation between hybrid performance and genetic divergence is dependent on the germplasm used. Rabbani et al. (2008) evaluated the genetic polymorphism and identities of several Asian rice cultivars by using random amplified polymorphic DNA technique. On the basis of analysis performed on similarity matrix by using UPGMA, they grouped 40 cultivars in to three main clusters correspondent to aromatic, non-aromatic and japonica group, and a few independent cultivars. The cluster analysis placed most of the aromatic cultivars close to each other showing a high level of genetic relatedness. But the clusters produced by the aromatic cultivars were distinct from those of non-aromatic and japonica types. In this study, several improved and obsolete cultivars originating from diverse sources did not produce well defined distinct groups and indicated no association between the RAPD patterns and the geographic origin of the cultivars used. Amita et al. (2005) performed molecular and hybridization studies to investigate variation patterns in O. meridionalis by producing 119 polymorphic RAPD markers from 12, 10-mer operon primers. In addition, they detected 67 alleles by using 11 SSR primers. They showed speciation in O. meridionalis a with respect to its geographic distribution in northern Australia and Irian Jaya. Santhy et al. (2003) test ed application of RAPD markers for the identification of three rice (Oryza sativa L.) hybrids and their parental lines i.e. CMS female parent (A line), maintainer (B line) and pollen parent (R line), using 17 random oligonucleotides. It was possible to distinguish each of these genotypes, following a combination of selected primers. The results are discussed in view of its application for the purpose of Plant Variety Protection and for testing the genetic purity of A line and hybrid seed lots. Simple Sequence Repeats Analysis Microsatellites or simple sequence repeats (SSRs) are simple tandemly repeated di- to penta-nucleotide sequence motifs. Microsatellite data are also commonly used to assess genetic relationships between populations and individuals through the estimation of genetic distances (e.g. Beja-Pereira et al., 2003; Ibeagha-Awemu et al., 2004; Joshi et al., 2004; Sodhi et al., 2005; Tapio et al., 2005). The most commonly used measure of genetic distances is Neis standard genetic distance (DS) (Nei, 1972). Because of microsatellite abundance and even distribution in nuclear genomes of eukaryotes and some prokaryotic genomes, they offer valuable good source of polymorphism, which make them a promising class of genetic markers. The high levels of polymorphism performed by these markers; they are mostly referred as SSLP (simple sequence length polymorphism). Li et al. (2004) examined genetic diversity within and differentiation between the indica and japonica subspecies, including 22 accessions of indica and 35 of japonica rice by using five microsatellite loci from each chromosome having total 60 loci. Evaluating on chromosome-based comparisons it is concluded that nine chromosomes (1, 2, 3, 4, 5, 8, 9, 10 and 11) harboured higher levels of genetic diversity within the indica rice than the japonica rice. By applying chromosome-based comparisons they suggested that the extent of the indica-japonica differentiation varied substantially, ranging from 7.62% in chromosome 3 to 28.72% in chromosome 1. At 15 of the SSR loci, traditional and crossbred Basmati rice varieties amplified different alleles than those in the indica and/or japonica rice varieties. During this study the identified SSR markers, which can be used to differentiate among the traditional Basmati varieties and between traditional Basmati and other crossbred Basmati or long grain, non-Basmati rice varieties. Genetic relationships among rice genotypes as determined by UPGMA cluster analysis and three-dimensional s caling based on principal component analysis showed that the three traditional Basmati rice varieties are closely related and have varying degree of similarity with other crossbred Basmati rice varieties Priyanka et al. (2004). Amanda et al. (2004) classified 234 accessions of rice into five distinct groups corresponding to indica, aus, aromatic, temperate japonica, and tropical japonica rices using 169 microsatellite markers. Yunbi et al. (2004) evaluated diversity in 236 rice accessions by applying 113 restriction fragment length polymorphism (RFLP) and 60 simple sequence repeat (SSR) loci at DNA level. Higher value of polymorphism information contents (0.66) was recorded for SSR markers as compared to RFLP (0.36). A diverse subset of 31 rice cultivars was identified that embodied 95% of RFLP and 74% of SSR alleles. This subset was useful in developing core collections and an efficient source of genetic diversity for future crop improvement. Zhang et al. (2005) evaluated the poten tial of discriminate analysis (DA) to identify candidate markers linked with agronomic traits among inbred lines of rice (Oryza sativa L.). A sum of 218 lines originating from the US and Asia were planted in field plots of Texas. Data were collected for 12 economically important traits, and DNA profiles of each inbred line were produced using 60 SSR and 114 RFLP markers. Model-based methods revealed population structure among the lines. Associated marker alleles pointed to the same and different regions on the rice genetic map when compared to previous QTL mapping experiments. Results of the study suggested that candidate markers associated with agronomic traits can be readily detected among inbred lines of rice. Bajracharya et al. (2005) estimated genetic diversity of rice landraces collected from different locations of Nepal based on agro-morphological variability and microsatellite marker polymorphism. They 39 microsatellite (simple sequence repeats, SSR) markers among these coll ected accessions by using 10 different names. After studying all these qualitative and quantitative traits they concluded that these accessions showed low morphological diversity having an average Shannon Weaver diversity index of 0.23. Among the studied traits only 16 morphological traits showed significant variation among the accessions. Discriminant function analysis showed that only 36% of accessions could be clustered according to name by morphological traits. Only one SSR locus was polymorphic, distinguishing only one accession. Genetic differences among new rice lines (NERICA), developed by cross breeding of African rice (Oryza glaberrima) with high yielding Asian rice (Oryza sativa subsp. japonica), were explored by using simple sequence repeat markers (Semagn et al. 2006). Michael et al. (2006) characterized 330 rice accessions, including 246 Indonesian landraces and 63 Indonesian improved cultivars, by studying 30 fluorescently-labeled microsatellite markers. By using gene tic diversity analysis they characterized the Indonesian landraces as 68% indica and 32% tropical japonica, having an indica gene diversity of 0.53 and a tropical japonica gene diversity of 0.56

Tuesday, August 20, 2019

Minority vs. Majority Government

Minority vs. Majority Government Minority vs. Majority Government Depending on who you are both a majority government and a minority government could either make or break the development of your country. A majority government is formed by one party who receives the majority vote over all other parties in the legislature. This government is able to pass legislation, make sure the House of Commons is able to function and can stay in power more easily then a minority government (Storm, 1999). Minority governments are single party that form a government without receiving the majority of seats in the legislature (Storm, 1999). A minority government has to make decisions with other parties and adjust policies to get enough votes from other parties to pass policies. They must constantly work to make sure the House of Commons is running smoothly to stay in power (Storm, 1999). Canadians struggle to decide which government is more productive and helpful to maintain the steadiness of our country. In Canada, we currently have a minority government which isnt working what we need is a strong majority government. On both sides of the political specturm they think its good to have minority governments, essentially because they cant get too much done (McKelvy, 2009). They believe that as long as we have a minority government we can do whatever we want and Canada will do just fine (McKelvy, 2009). This is a skeptical and pessimistic view of how the federal governments role is potrayed. The major weakness of minority a government is their tendency to last for only a short period. Governments are often unable to fully pursue their policies because other partys will collapse before they can negotiate anything.. The average duration of minority governments in Canada is approximately 18 months (Gray, 2008). Minority governments have to negotiate with other parties regularly to get policies passed and it is difficult to bring consistency to government policy because its hard to get everyone to agree on policies. The party in charge may have to compromise with one party on one issue and then compromis e with another party on a different issue (McKelvy, 2009). This can be a problem because government actions in one area can neglect government actions in another area. Inefficiency and the demand for negotiation can slow down the government immensely. Instead of being able to take swift action on demanding issues, governments are forced to engage in long and dull negotiation with other political parties (McKelvy, 2009). This can cause issues that require immediate action, such as policies and the budgets. Minority governments open the possibility for less responsibility (McKelvy, 2009). This is because the different parties are working together in making government policy. It can be difficult to locate blame when policy goes wrong, as the different parties may simply blame one another. Long term success requires long term ideas and this is something, especially Harpers minority government, cannot achieve because a minority government only lasts for a short period of time, so no decisions can be made (Collage, 2008). A minority government of Liberal, NDP or Conservatives is not acceptable. With a majority government they will be able to pass legislation with no problems and there will be more government intervention (Collage, 2008). The Prime Minister and the Cabinet have a lot of control over the government plans and policies. They can put forth whatever policy they like, and then they can ensure that those policies are supported in the House of Commons and then they can be passed into law (Collage, 2008). A majority government could formulate a plan that would be able to take action and a minority government would end up debating all day about how effective such actions might be instead of taking action. Canadians need a strong majority government ensure us of a Parliament that will work when its needed. Prime Minister Stephen Harper is our current prime minister and wants a majority government the next time the Canadians go to the polls for a federal election (Gray, 2008). Most Canadians are unsatisfied with Harper as their minority government and believe he would do poorly running under a majority government (Gray, 2008). Under a minority government Harper has been unable to do certain things that a majority government would be able to do and others would say he is just unfit to govern. The current minority government has caused the Parliament to become increasingly dysfunctional. Harper is governing like he has the majority and is trying to bully the opposition which is hurting the economic crisis we are in (Millard, 2009). Harper wanted to score political points by trying to eliminate public financing of political parties. That caused the opposition leaders to consider attempting to replace the government by forming a coalition. Harper could have cut public financing by more tha n half from 1.95 per vote to 0.95 per vote (Millard, 2009). This would have been the sensible thing to do in the current economic environment. A minority government wont work if all of the parties dont work together. Harper made a mistake by appointing people to the senate when he said he wouldnt. Harper promised to reform the senate and promised not to appoint any unelected Senators (Hogan, 2010). He acted hypocritically in appointing senators he said in the past he would not. He broke a promise he made and all the prime ministers promised they would not appoint any senators either. This shows that Harper is unable to keep a promise which means he could break any of his other ones. Its harder running under a minority government because not only does Harper have to keep promises to the country, but he also has to keep promise to his opposing parties. Harper also refused to bail out failed industries after he gave more than 3 billion to rescue the auto sector (Ross, 2008). Harper said he would offer similar aid to the struggling mining and forestry industries in Januarys next month budget (Ross, 2010). There was no mention of support for resource industries in Harpers recent Throne Speech or in i ts fiscal update last month. This provoked so much criticism that it led to the creation of the opposition collation and almost cost Harper its minority government. Canada is sinking under Harpers debt even though has stated that he would not engage in deficit financing nor take on new debt (Valpy, 2009). According to Harper pump priming is a solution to the weakness in our economy (Valpy, 2009). Harper said he is going to spend tens of billion of dollars to try and pump money into the economy and Canada will have to endure large deficits along the way. In the 2008 election Harpers conservative government was already spending its way deeper into debt. A massive amount of 89.5 billion dollars was produced of financial requirement deficit (Valpy, 2009). By 2009 the federal government had already racked up 79.5 billion in new debt financing (Valpy, 2009). A deficit of $33.7 billion for the 2010 fiscal year and $29.8 billion the following year is predicted (Valpy, 2009). Looking at the Conservatives report and observing their forecasts of budget deficits, it seems like these numbers could turn out to be bigger. Harper was predicting small surpluses for years coming and then predicted enormous ones. That leads me to believe these deficits could turn out to be much larger that we think. If the economy worsens beyond what is expected, this could end up being true. Canadians are fed up with the repeated minority governments that keep occurring in the House of Commons. According to a poll conducted by Harris Decimal, 64 percent of Canadians want a majority government and 36 percent want a minority government (Howard, 2009). Even the people believe that their country is better off with a majority government then a minority government. It doesnt look like Canada will be getting a majority government anytime soon due to the Bloc Quebecois party. Gilles Duceppe argued that a Conservative majority would be bad for Quebec and only his party can stop that from happening (Howard, 2009). The Bloc Quebecois party wants to stop a majority government from occurring by getting everyone in Quebec to vote for there party which will not allow Canada to be under a majority government. Harper said â€Å"if the Conservatives dont succeed in getting a majority he predicted the Liberals will govern in a coalition with the NDP and Bloc Quebecois† (Howard, 200 9). â€Å"If they get together and force us to the polls, we have to teach them a lesson and get back there with a majority, and make sure their little coalition never happens, Harper said. (Howard, 2009) The Liberals claim that this shows that Harper doesnt want to work with the other parties in Parliament (Howard, 2009). I see a coalition government of the NDP and Bloc as a desperate grab for power; I think its also a reflection of the genuine frustration with the current hopelessness of our government. The Liberal party have said they want to make sure that the Conservative party is not in power anymore (Howard, 2009). This shows that a majority government will never work; we need one person governing our country. We will never have a minority government if ever party is not working together because the population will disperse themselves to one party and no party will ever get the majority votes. Instead of continuing with the governments approach to Senate reform, we should try to create a body of government that can really watch the House of Commons and monitor what is happening. We could do this by using a system of proportional representation to elect members and to create a Senate that has equality and an effective body that would provide a number of benefits (Kline, 2009). First, it would allow a different diversity of parties to be able to represent themselves in Parliament (Kline, 2009). Votes that are wasted on small parties like the Greens or Bloc Quebecois could give their support to majority governments in the House of Commons and also giving small parties a voice in the Senate. Second, it would allow the Prime Minister and the Cabinet to run the country as a dictatorship if they were given the majority in the House of Commons (Kline, 2009). This would mean a less interfering government because it would make it harder for the Parliament to pass legislation on imm ediate impulse and it would allow Canadians to have a greater say in the future of their country. Furthermore, what our country needs is a majority government. Minority governments get nowhere because the opposition parties and the prime minister are always bullying each other. As I have stated in my essay Stephan Harper has caused a lot of problems because he is ruling as a majority government even though he is running under a minority government. Canadian majorities are clearly more productive legislatively than minorities. According to the history of past government the rate that bills were passed in a majority government were 20 per cent higher than in minority governments (Gray, 2009). Majority status appears to have a significant effect on both productivity and success. Canadians are growing tired of minority governments, so why is it that we keep voting for different political parties where we are unable to obtain a majority government? References Gray, John. Majority or minority government, which is actually more productive?. Canada Votes. CBC, 26 Sep 2008. Web. 22 Mar 2010. http://www.cbc.ca/news/canadavotes/story/2008/09/24/f-minority.html Hogan, James. CBC News. Harper names 5 to Senate. N.p., 29 Jan 2010. Web. 23 Mar 2010. Howard, Cody. CTV News. Canadians grow weary of minority governments. CTV globe media, 13 July 2009. Web. 22 Mar 2010. http://www.ctv.ca/servlet/ArticleNews/storyv2/CTVNews/20090713/minority_govenment_090713?s_name=no_ads= Kline, Jesse. The Shotgun Blog. Canadians want a majority government. N.p., 07 May 2009. Web. 24 Mar 2010. McKelvy, Andrew. Minority Government in Canada: Impediment to, or Impotence Against, the Governments Legislative Agenda?. Conference Papers Midwestern Political Science Association (2009): 1. Academic Search Premier. EBSCO. Web. 22 Mar. 2010. Millard, Gregory. Stephen Harper and the Politics of the Bully. Dalhousie Review 89.3 (2009): 329-336. Academic Search Premier. EBSCO. Web. 22 Mar. 2010. Robin, Collage. These times call for a majority government. Macleans 13 Oct. 2008: 4. Academic Search Premier. EBSCO. Web. 22 Mar. 2010. Ross, Laura. CTV News. Harper McGuinty announce $4B auto bailout package. N.p., 20 Dec 2008. Web. 23 Mar 2010. Storm, Kaare. Government and Majority Rule. New York: Cambridge University Press, 1999. 45-90. Valpy, Michael. Globe and Mail. Ignatieff blasts Harpers deficit games. N.p., 23 Jan 2009. Web. 23 Mar 2010.

Monday, August 19, 2019

Problems of Medieval Europe :: European Europe History

Problems of Medieval Europe The setting: Medieval Europe. The problem: the pope is living in Avignon, under strict control from the French King. The plague is ravaging Europe, leaving behind whole cities of corpses. Sanitation is very poor, there are no sewer systems, and more often than not, one could find human and animal feces lining the streets. The standard of living is very low, and much of this is blamed on religion. Many people would like to see the pope dead. Solutions are virtually non-existent. The pope is looking for a way to restore his power, and improve the life of Europeans. The main problem facing the pope was, of course, the plague. Nearly twenty-five million people had died of this highly infectious disease already, and it didn't appear to be slowing. Medieval physicians had developed a number of "cures," some as absurd as placing live chickens on the wounds of the infected. Due to the primitive technology at that time, there were very few actual cures. Many of the practices of the doctors were invented simply to deceive the populous into believing that they had cures, and that all was not lost. The pope, in his quarters at Avignon, sat between two large fires. They thought that this would purify the "bad air" which most blamed for the spread of the plague. Although there was no bad air, the fires actually did prevent the plague, killing off the bubonic bacteria. This was an example of what some people call "accidental science," or a discovery made from superstition, or by accident. From the viewpoint of a medieval doctor, there were few things you could do. Most medicine at that time was based on the four humors, and the four qualities. The four humors were phlegm, blood, bile, and black bile. Illness would occur when these humors were imbalanced. Doctors often let blood, attempting to restore balance. There were also four qualities; heat, cold, moistness, dryness. Diseases were often deemed to have two qualities, i.e. hot and dry. If a person had a disease that was hot and dry, they would be administered a plant that was considered cold and moist. Basically what I have tried to say in the previous two chapters is that there was no medicinal cure for the plague in medieval times. If they had antibiotics, however, there would have been very few fatalities.

Sound in The Tempest and the New Orthodox View :: Tempest essays

Sound in The Tempest and the New Orthodox View  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   Critics have offered varying evaluations of the characters in William Shakespeare's The Tempest. Some consider Prospero to be magnanimous for forgiving his enemies, for freeing Ariel from the confines of a tree, and for treating Caliban with great sympathy until the monster's attempted rape of Miranda. Others view Prospero as an oppressive colonizer and consider both Caliban and Ariel to be his innocent and mistreated subjects. In his article "Reading The Tempest," Russ McDonald argues that the new orthodox interpretation of The Tempest, "which exalts the colonized, is as narrow as the old, which idealizes and excuses the colonizer" (117). He argues that the actual status of the characters is considerably more ambiguous, and he supports his view by analyzing the rhetorical devices present in the play. However, a close examination of the various sounds disbursed throughout the work--including speech, silence, and music--tends to support a less ambiguous view of the characters. Indeed, it tends to lend support to the new orthodox view that Prospero is an oppressive colonizer, for he often threatens his enemies and servants with unpleasant sounds and demands silence from others, including his daughter. The play begins with a ship's crew being subject to terrifying sounds that Prospero has ordered Ariel to produce. The sounds are all loud: "whistle," "storm," "cry," "thunderclaps," "fire and cracks," and "roaring" (1.1.7, 14; 1.2.203-5; 2.1.2). The terror that these sounds and the accompanying storm inflict upon the mariners is evidenced by their cries: "All lost! To prayers! To prayers! All lost!" (1.1.52). The infliction of these sounds is also made to appear unjust when Miranda pleads with her father: "If . . . you have / Put these wild waters in this roar, allay them. / . . . O, the cry did knock / Against my very heart. Poor souls, they perished!" (2.1.1-9). Indeed, Prospero often refers to unpleasant sounds as a means of threatening others. "I will plague them all, / Even to roaring," he says of Caliban, Trinculo, and Stephano (4.1.188-214). When Prospero believes Ariel is not providing an eager and willful service, he threatens the spirit with imprisonment in a tree, reminding Ariel that when he was previously trapped, his "groans / Did make wolves howl" (1.2.289-90). Prospero also tells him, "Thou hast howled away twelve winters" (1.2.298). Similarly, Prospero threatens Caliban, carrying out his threats and subjecting the monster to tortures accompanied by unpleasant sounds. Sound in The Tempest and the New Orthodox View :: Tempest essays Sound in The Tempest and the New Orthodox View  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚  Ã‚   Critics have offered varying evaluations of the characters in William Shakespeare's The Tempest. Some consider Prospero to be magnanimous for forgiving his enemies, for freeing Ariel from the confines of a tree, and for treating Caliban with great sympathy until the monster's attempted rape of Miranda. Others view Prospero as an oppressive colonizer and consider both Caliban and Ariel to be his innocent and mistreated subjects. In his article "Reading The Tempest," Russ McDonald argues that the new orthodox interpretation of The Tempest, "which exalts the colonized, is as narrow as the old, which idealizes and excuses the colonizer" (117). He argues that the actual status of the characters is considerably more ambiguous, and he supports his view by analyzing the rhetorical devices present in the play. However, a close examination of the various sounds disbursed throughout the work--including speech, silence, and music--tends to support a less ambiguous view of the characters. Indeed, it tends to lend support to the new orthodox view that Prospero is an oppressive colonizer, for he often threatens his enemies and servants with unpleasant sounds and demands silence from others, including his daughter. The play begins with a ship's crew being subject to terrifying sounds that Prospero has ordered Ariel to produce. The sounds are all loud: "whistle," "storm," "cry," "thunderclaps," "fire and cracks," and "roaring" (1.1.7, 14; 1.2.203-5; 2.1.2). The terror that these sounds and the accompanying storm inflict upon the mariners is evidenced by their cries: "All lost! To prayers! To prayers! All lost!" (1.1.52). The infliction of these sounds is also made to appear unjust when Miranda pleads with her father: "If . . . you have / Put these wild waters in this roar, allay them. / . . . O, the cry did knock / Against my very heart. Poor souls, they perished!" (2.1.1-9). Indeed, Prospero often refers to unpleasant sounds as a means of threatening others. "I will plague them all, / Even to roaring," he says of Caliban, Trinculo, and Stephano (4.1.188-214). When Prospero believes Ariel is not providing an eager and willful service, he threatens the spirit with imprisonment in a tree, reminding Ariel that when he was previously trapped, his "groans / Did make wolves howl" (1.2.289-90). Prospero also tells him, "Thou hast howled away twelve winters" (1.2.298). Similarly, Prospero threatens Caliban, carrying out his threats and subjecting the monster to tortures accompanied by unpleasant sounds.

Sunday, August 18, 2019

Noncontradiction as a Scientific Modus Operandi :: Science Logic Papers

Noncontradiction as a Scientific Modus Operandi We explicate the view that our ignorance of the nature of the real world R, more so than a lack of ingenuity or sufficient time to have deduced the truth from what is so far known, accounts for the inadequacies of our theories of truth and systems of logic. Because of these inadecuacies, advocacy of substantial correctness of such theories and systems is certainly not right and should be replaced with a perspective of Explorationism which is the broadest possible investigation of potential theories and systems along with the realization that all such theories and systems are partial and tentative. For example, the position of classical logic is clearly untenable from the perspective of explorationism. Due to ignorance regarding R and, consequently, the partial and evidential nature of our knowledge about R, an explorationist foundational logical framework should contain machinery which goes beyond that of classical logic in the direction of allowing for the handling of confirmatory a nd refutatory evidential knowledge. Such a foundational framework (which I call Evidence Logic) is described and analysed in terms of its ability to tolerate substantial evidential conflict while not allowing contraditions. 0. Overview The variegated landscape of theories of truth and systems of logic, wherein each is cogently argued while yet inconclusive, is substantially accounted for by the fact that we just don’t know enough yet about the nature of our universe, let us call it R, to be able to settle on one or the other of these theories and systems as adequate for the representation and processing of our knowledge about R. In this paper firstly we discuss this thesis, that it is primarily our ignorance of R, and not any failure to rigorously construct our theories and systems, that is a fundamental cause of the inadequacies of these theories and systems. Secondly we will delineate a scientific perspective, Explorationism, which, if the thesis first considered is correct, is deserving of advocacy. Finally, we exemplify this perspective by exhibiting a logic, Evidence Logic (EL), which incorporates a broadened concept of negation which (1) provides for the representation and processing of both confirmato ry and refutatory evidential knowledge including the possibility of a generous range of conflicting evidence while yet (2) enforces noncontradiction. 1. The inadequacy of our theories of truth and systems of logic Any survey of the gamut of theories of truth so far constructed makes clear that, while each may be presented cogently, each manages to tell only part of the story.

Saturday, August 17, 2019

Working Capital Analysis

CHAPTER-I INTRODUCTION 1. 1 Back ground of Study Working Capital refers to that part of the firm’s capital, which is required for financing short-term or current assets such a cash marketable securities, debtors and inventories. Funds thus, invested in current assets keep revolving fast and are constantly converted into cash and this cash flow out again in exchange for other current assets. Working Capital is also known as revolving or circulating capital or short-term capital. Therefore, working capital management is the same of liquidity management and its relate inversely with profitability.It is significance for any industries due to the investment in Current Assets (CA) must be adequate because inadequate or excessive inadequate working capital can disturb production and can also threaten the solvency of firm, if it fails to meet its current obligation excessive investment in CA should be avoided, since it impairs firm's profitability Secondly, need for working capital ar ises due to increasing level of business activity ; it is to provided quickly some time surplus fund may arises which should be invested in Short term securities , they should not be kept idle.The importance of Working capital management compelled to the firms to try the optimal level of investment in each element such as inventories, cash, account receivables but the firm also consider to way of financing the current assets. This means, consideration of current liabilities which include account payables, notes payable, interest payable and other shot-term debt.In addition, the firm can adopt an aggressive working capital management policy with a low level of current assets as a percentage of total assets, or it may also be used for the financing decisions of the firm in the form of high level of current liabilities as a percentage of total liabilities(Nazir and Afza,2009), and it is the opposite in conservative working capital management policy. On the other hand, it should be dist inguished between three policies that related directly with the working capital efficiency.First policy is collection policy, that measured by average receivables collection period (ARCP) which is meaning the average length of time required to convert the firm receivables into cash. Second policy is inventory policy, which expressed by average conversion inventory period (ACI). It means the average length of time required to convert raw materials into finished goods and then sell these goods.Third policy of working capital efficiency is payment policy, which measured by average payment period (APP) that means the length time between the purchase of materials and the payment of cash (Weston and Brigham,1993). These policies require from company to accelerate the collections of receivables, accelerate its inventory, accelerate the payment cycle, and reduce the cost of the working capital needs.Above mentioned policies can be merged them in one general policy, is called cash conversion cycle (CCC) developed by Richards and Laughlin(1980) which focuses on the length of time between when the firm makes payments and when it receives cash inflow. To fulfill the one of the most important goal of organization to maximization of share holder’s wealth of a firm is possible only when there is sufficient return from the operations and successful sales activity is necessary for earning profit sales without convert into cash immediately.To generate the sales and revenue activities there will be the s invisible time lap between the sale of good and receipt of cash. Hence, the time taken to convert raw material into cash is known as operating cycle that includes following activities in different phase. At first phase: * Conversion of cash into raw material * Conversion of raw material into work in progress * Conversion of Work in progress into finished goods * Conversion of finished goods into Sales ( Debtors and cash) At second Phase: Cash received and at third phase i s payment of credit.A low cash conversion cycle allows the managers to minimize holdings of relative unproductive assets such as cash and marketable securities, preserves the firm’s debt capacity since less short-term borrowing is required to provide liquidity and corresponds to a higher present value of net cash flows from firms assets Moreover, the cash conversion cycle is an important technique of analysis for the financial mangers of firm to assess why and when the firm needs more cash to sustain its activities. I am going to comparative study of Surya Nepal Private Limited (SNPL) is an Indo-Nepal-UK joint enture, which started operations in Nepal in 1986. SNPL, a subsidiary of ITC Ltd, India, is the largest private sector enterprise in Nepal. The balance shares are held by dispersed Nepalese shareholders and British American Tobacco, UK. Surya Nepal's businesses include manufacture and marketing of cigarettes and readymade garments in Nepal as well as exports of ready-ma de garments with a total turnover of over US $100 million. Secondly, The guiding force behind Dabur's growth and success has been the wealth of nature and its limitless capacity to support life.And we have constantly taken care to preserve and protect this natural bounty. With this overall vision of and to eco-sustenance, expand Dabur's resource and production base, Dabur Nepal Private Limited was set up as an independent Group company in 1992. This new company, set amidst the verdant greens and towering mountains of the Himalayan kingdom of Nepal, has established a unique bond of technology and preservation. 1. 2 Problem of Statement The management of a company's working capital significantly influences its profitability. In the short term, companies risk being short on liquidity if the working capital level deteriorates.In the long term, too much working capital lowers the return on investment and reduces the value of the company. In contrast, a reduction of the working capital ca n significantly improve cash flows and free up capital from a company's balance sheet. This capital can then be used to reduce debts, pay dividends to investors or reinvest in company growth. In the context of Nepal there is not practically implementation of working capital management technique that can brings the liquidity problem in short term and solvency problem in long term due to loss on business.This can be the one most important reason for the lower growth rate of manufacturing firm. I want to gain insight into this field and to identify potential areas for optimization of working capital management for the profitability on the Nepalese manufacturing firm. Performance of firm on the topic of working capital management is very essential to reach the optimum level of working capital then to enhance their profitability. But these elements can be affected by Nature of business, seasonality of operations, production policy, market condition, and political scenario.Therefore, I ha ve done this investigation to know the answer of following question. a. What are the factors of working capital for Nepalese manufacturing firm? b. How can working capital affects the performance to enhance profitability of firms? c. How is the performance of firm to achieve the optimal working capital in order to maximize the profitability? 1. 3 Objective of the Study The main objective of the research is to measure the impact of working capital management on the profitability for Nepalese manufacturing firm. The specific objectives of the study are summarizing as following. a.To analyze the relationship between working capital management and profitability for manufacturing firm. b. To determine the relationship between size of firm and the profitability c. To Know the relationship between leverage and profitability. 1. 4 Limitation of the Study This study is intended to measure the impact of working capital management on profitability of Nepalese manufacturing firm but the study a lso influences from the following limitation. a. There isn't financial sponsor for the depth study. b. Due to the time constraints it is not possible to analyze the each variable in details. c.In depth analysis and the study of financial position is not feasible because of the policy and privacy of firm. d. The information is assuming true that is taken from different source. CHAPTER-II LITERATURE REVIEW 2. 1 Literature Review According to Wilner (2000) most firms extensively use trade credit despite its apparent greater cost, and trade credit interest rates commonly exceed 18 percent and Deloof (2003) also found that according to National Bank statistics during 1997, Belgian companies had accounts payable of only 13% of the total asset and accounts receivable and Inventory of 17% and 10% of the total asset respectively.Singh and Pandey (2008) discussed the impact of working capital management in the profitability of Hindalco Industries Limited. Regression results showed that curren t ratio, liquid ratio, receivable turnover ratio and working capital to total assets had statically significant impact on profitability. Dong and Su (2010) examined the relationship between profitability, the cash onversion cycle and its component for listed firms in Vietnam stock market for period (2006-2008). They resulted that there is strong negative relationship between cash conversion cycle and the profitability.Cote and Latham (1999, p. 261) argued that management of receivables, inventory and accounts payable have tremendous impact on cash flows, which in turn affect the profitability of firms. According to Long, Malitz and Ravid (1993) it is seen that liberal credit terms to the customers increase the sales level of the firm, though having a continuous troubleshooting with managing short term financing in the finance department. The decision lays with the firm which one to put more importance on. Scherr (1989, p. 6) claimed that companies can strengthen strong cash flow lev els, improve profitability, budgeting and forecasting process, predictability and manageability of results, heighten risk if they implement the best practices in working capital. Amit, Sur and Rakshit (2005) studied the relationship between working capital and profitability in the context of Indian pharmaceutical industries and concluded that no definite relationship can be established between profitability and liquidity. Cote and Latham (1999, p. 61) argued that management of receivables, inventory and accounts payable have tremendous impact on cash flows, which in turn affect the profitability of firms. Scherr (1989, p. 16) claimed that companies can strengthen strong cash flow levels, improve profitability, budgeting and forecasting process, predictability and manageability of results, heighten risk if they implement the best practices in working capital. Eljelly(2004) identified the relation between profitability and liquidity who was examined, as measured by current ratio and c ash gap (cash conversion cycle) on a sample of joint stock firms in Saudi Arabia.The study found that the cash conversion cycle was of more importance as a measure of liquidity than the current ratio that affects profitability. The size variable was found to have significant effect on profitability at the industry level. The results were stable and had important implications for liquidity management in various Saudi firms. First, it was clear that there was a negative relationship between profitability and liquidity indicators such as current ratio and cash gap in the Saudi sample examined. Second, the study also revealed that there was great variation among industries with respect to the significant measure of liquidity.Sur Biswas and Ganguly (2001) revealed in their study of Indian aluminium producing industry, a very significant positive association between liquidity and profitability. All previous studies had reached to the same results approximately, which had proved there is t he negative relationship between the working capital, debt ratio, current ratio and profitability, and the positive relationship between size of the firm with profitability. This study tries to depend on previous studies to provide new evidence on how working capital can effect on the profitability. . 2 Research Frame Work Model ROA Leverage Performance Working Capital Efficiency Profitability of the Firm Size of Firm Current Liabilities Current Assets Organization Planning Growth of business Ln of Sales Debt ratio Liquidity ratio: CR All the components such as Working Capital, Profitability and Size of the firms, Liquidity, and Leverage performance are interrelated to each other. The working capital affects the profitability of the firm. Similarly size, leverage, and liquidity affect the working capital requirement and profitability of the firm.If there is low in current assets then it can’t pay the short term obligation and if firms keep in high ratio then investment opport unity will lose that decreases the value of profitability elements such as ROE and ROA. Secondly, if there is high concentration on sales by keeping low liquid assets then profit can increase and it helps to increase the growth rate of company and fulfill the objective of shareholder’s wealth maximization and ease for the competition but low liquid assets can creates the risk of liquidity. Therefore, all components of above mentioned are interrelated positively and negatively.After analyzing the financial ratio BOD, manager can formulate the policy for sustainable business as well as investors will take best decision for the investment. This study has been guided according to the above variables and discussed the variables relation after studied of two firms in detail in the below. Hence, this study will benefit for the best decision of working capital requirement to manage the profitability, leverage in long term and to growth the firm in stable rate. CHAPTER-III DATA COLLEC TION AND METHODOLOGY 3. 1 Research Data CollectionThe data has taken from the secondary source regarding to the official site of Surya Nepal Pvt. Ltd and Dabur Nepal Pvt Ltd. Secondary data is assumed as an enough and reliable. Sample of this study has been focused on the joint venture Nepalese manufacturing firms. These two firms have chosen as a sample company due to big market in Nepal. To fulfill the objective of research, report is prepared by taking a financial data of two sample companies from 2006 to 2011. 3. 2 Definition of variable I have used of dependent and independent variables to complete the study are as below.Dependent variables include profitability measure which will be computed by the following equation: Return on Assets (ROA)=Net Operating IncomeTotal Assets Secondly, independent variables have been divided in two parts. First part includes working capital management variables. †¢ Average receivable collections period (ARCP) are used to express the credit p olicy. It is calculated by using following equation: Average receivable collections period (ARCP)=Account Receivables *365/Sales †¢ Average conversion inventory period (ACIP), which is expressed the inventory policy.It will be identified by following formula: Average conversion inventory period (ACIP)= Inventory *365/Cost of Sales †¢ Average payment period (APP) is used to reflect the payment policy it is measured measured by following equation: Average payment period (APP) =Accounts Payables *365/Cost of Sales †¢ Cash conversion cycle (CCC) is used to express the overall impact on working capital efficiency, and that is calculated by using following equation. Cash Conversion Cycle (CCC)=ARCP+ACIP-APP At the second phase of independent variables has been included as below. †¢ Size of the company = Natural of logarithm of sales (LNS). Current ratio (CR) = Current assets/Current Liabilities. †¢ Financial leverage ratio (FL) = Total Liabilities / Total Assets. 3. 3 Empirical Analysis This section contains the descriptive analysis by taking the help of mean, standard deviation, maximum and minimum value of all variables that is used in study. Similary, on the second phase of analysis here has been explained the relationship between the variables by using correlation coefficient. Moreover, regression model has been used to quantify the relation between variable and to measure the accuracy of this report.Multiple regression models have used to complete the regression analysis. All types of analysis and graphical representation will be expressed by using the MS office package 2007. For this study I have used 4 regression models to quantify the relation and model is as below. 1. ROA= a+b1ARCP+b2CR+b3FL+b4LNS (model -1) 2. ROA= a+b1ACIP+b2CR+b3FL+b4LNS (model-2) 3. ROA= a+b1APP+b2CR+b3FL+b4LNS (model-3) 4. ROA= a+b1CCC+b2CR+b3FL+b4LNS (model-3) CHAPTER-IV DATA ANALYSIS AND PRESENTATION 5. 1. Empirical Analysis and Findings 4. 1. 1Descriptive An alysis: Dabur Nepal Pvt. Ltd (Table 1) | ROA| ARCP| ACIP| APP| CCC| CR| FL| Lns|   |   |   |   |   |   |   |   | Mean| 0. 034| 35. 67| 75. 12| 42. 35| 68. 44| 2. 00| 0. 248| 21. 73| Standard Deviation| 0. 028| 19. 74| 31. 74| 35. 27| 39. 24| 0. 676| 0. 055| 0. 346| Minimum| 0. 001| 4. 13| 17. 37| 8. 33| 6. 30| 1. 52| 0. 207| 21. 39| Maximum| 0. 084| 55. 23| 111. 78| 93. 46| 120. 94| 3. 19| 0. 351| 22. 38| Count| 6| 6| 6| 6| 6| 6| 6| 6| According to the above table, ROA on average is 34% and ROA existed between 0. 1 % to 0. 84%. The average receivables collection period has 5 days (approximately) as minimum to collect its receivables from the purchasers but it takes 55. 23 days as maximum to collect its receivable.The average days of generating its sales on account about 35. 67 days. In addition, the average conversion inventory period (ACIP) takes about 17. 37 days to sell all its inventory as minimum and takes 111. 78 days as maximum. The mean days to sell the inven tories are 75. 12 days with standard deviation of 31. 74 days. About the APP, the firm has a minimum time 8. 33 days to pay its purchases on account and 93. 46 days as a maximum time. It takes an average 42. 35 days to pay its purchase with standard deviation of 35. 27. The cash conversion cycle (CCC) has 6. 30 days as a minimum time and maximum is 120. 94 days.The minimum current ratio (CR ) of the firm is 1. 52 and maximum is 3. 19 with the standard deviation of 0. 55%. The Natural Logarithm of size (LNS) shows minimum sales is 21. 39 and maximum is 22. 38 with the average of 21. 73. About the financial leverage is 20% as minimum and maximum is 35% with the standard deviation of 0. 55%. Surya Nepal PVT. LTD (Table 2) | ROA| ARCP| ACIP| APP| CCC| CR| FL| LNS| |   |   |   |   |   |   |   |   | Mean| 0. 287| 5. 11| 191. 55| 63. 45| 133. 21| 1. 72| 0. 424| 22. 82| Standard Deviation| 0. 086| 2. 90| 21. 84| 5. 88| 19. 96| 0. 678| 0. 152| 0. 339| Minimum| 0. 178| 2. 54| 170. 51| 57. 0| 110. 72| 1. 06| 0. 255| 22. 42| Maximum| 0. 389| 9. 86| 232. 04| 73. 16| 167. 62| 2. 44| 0. 594| 23. 31| Count| 6| 6| 6| 6| 6| 6| 6| 6| an average return on assets(ROA) is just 28% which is lower than Dabur Nepal Ltd. But the minimum and maximum value of ROA exists between 17 % to 38 % and less variability comparison with Dabur Nepal Ltd. The Average receivable collection period (ARCP) is 5. 11 days approximately and lower than Dabur. Thus, collection capacity of dabur is very strong. similarly, ACIP of Surya Nepal exist between 170. 51 days to 232. 04 days. About the average CCC of Surya Nepal is 133. 1 days which is higher than Dabur Nepal. Therefore, we can say that, cash inflow days in the Dabur Company are quicker than Surya Nepal. Moreover, an average 42% portion is existed under the total assets of the Surya firm that is higher than Dabur Company. It means, Surya Nepal takes more loans for the business. About the average size of Surya Nepal is 22. 82 that is h igher than Dabur. Graph: 1 source Table 1 and 2 Let summarize the above result: a. ROA of Surya Nepal is higher that tends to the meaning of; profitability volume is good rather than Dabur Nepal Pvt. Ltd. b. Credit collection capacity is stronger of Surya Nepal Pvt.Ltd. c. Surya Nepal Ltd. takes more time to convert the goods in raw material. d. Surya Nepal pays to supplier at delay comparison with Dabur. e. CCC of Surya is higher due to higher in ACIP and APP. 4. 1. 2. Correlation Coefficient Analysis Dabur Nepal Ltd. (Table 3) | ROA| ARCP| ACIP| APP| CCC| CR| FL| Lns| ROA| 1|   |   |   |   |   |   |   | ARCP| -0. 903| 1|   |   |   |   |   |   | ACIP| -0. 666| 0. 537| 1|   |   |   |   |   | APP| -0. 074| 0. 141| 0. 705| 1|   |   |   |   | CCC| -0. 927| 0. 811| 0. 446| -0. 258| 1|   |   |   | CR| 0. 835| -0. 627| -0. 888| -0. 450| -0. 6291| 1|   |   | FL| -0. 095| 0. 285| -0. 007| -0. 152| 0. 2743| 0. 48| 1|   | Lns| 0. 144| -0 . 172| 0. 348| 0. 609| -0. 352| -0. 203| -0. 572| 1| Surya Nepal Pvt. Ltd (Table 4) | ROA| ARCP| ACIP| APP| CCC| CR| FL| LNS| ROA| 1|   |   |   |   |   |   |   | ARCP| -0. 420| 1|   |   |   |   |   |   | ACIP| 0. 393| -0. 174| 1|   |   |   |   |   | APP| -0. 359| -0. 402| 0. 441| 1|   |   |   |   | CCC| -0. 263| 0. 073| 0. 939| 0. 130| 1|   |   |   | CR| -0. 892| 0. 493| -0. 443| -0. 736| -0. 196| 1|   |   | FL| -0. 869| -0. 457| 0. 541| 0. 769| 0. 299| -0. 992| 1|   | LNS| 0. 958| -0. 437| 0. 569| 0. 464| 0. 423| -0. 893| 0. 902| 1| According to the Table 3 and 4, Return on Assets (ROA) has negative relationship with ARCP.It tends to the meaning of longer the time of collection days reduces the profitability of firm. Therefore, if a firm reduces the length between sales and collection, it will increase the profitability through reinvest collections in profitable investments. Correlation results related negatively between the a verage conversion conversion inventories (ACIP) and ROA significantly in the case of Dabur. It means when the firm reduces the length time required converting raw material in to finished goods and then to sell those goods that lead to enhance profit.But in the case of Surya Nepal, there is positive relationship between ARCP and ROA. It means, it should take more stock for the high profit. The average Payment Period (APP) has negatively correlation with Profitability. It means, if the both firm shorten the length time between purchases goods and payment of the value of goods, it will lead to increase profitability. There are negative relationship between cash conversion cycle (CCC) and ROA. If the firms shorten its conversion cycle as much as possible without hurting its operation, it will reflect positively on profitability.Correlation coefficient of the size (LNS) firm is positive relationship with profitability that indicates if the firm increases its size of sales; it will lead t o increase its profitability. Current ratio refers to liquidity of the firm which relates positively correlated with ROA in case of Dabur but negatively correlated with ROA in case of Surya Nepal. Generally, if the firm invests its liquidity very well, it will generate high return and as per situation there might be required or not for holding of stocks in long term. About the Financial leverage that is negatively correlated with profitability.It means, if the firm depends on the financial leverage as much as need, ti carry itself financial obligation such as interest payment and principal payment and then it reflects negatively on its profitability. Dabur Nepal Pvt. Ltd. (Table 5) Year| 2006| 2007| 2008| 2009| 2010| 2011| ROA| 0. 031| 0. 030| 0. 022| 0. 001| 0. 084| 0. 038| CCC| 85. 74| 86. 30| 63. 87| 120. 93| 6. 29| 47. 46| Source Table 5. Graph 2 Conclusion: According to the above finding, the increasing in the value of CCC that decreases the value of ROA. And it is proved that there is negative relationship between ROA and CCC.Surya Nepal Pvt. Ltd Graph 3. Conclusion: In the case of Surya Nepal Pvt. Ltd, there is positive relationship between ROA anc CCC. This means to increase the profit of Surya firm then they have to increase the value of working capital component. 4. 1. 3Regression Analysis: Dabur Nepal Pvt. Ltd Table 6 Independent Variables| Model-1| Model-2| Model-3| Model-4| ARCP| -0. 001|   |   |   | ACIP|   | -0. 020|   |   | APP|   |   | -0. 0003|   | CCC|   |   |   | -0. 0004| CR| 0. 020| 0. 115| 0. 045| 0. 017| FL| -0. 021| -0. 577| -0. 178| -0. 015| Lns| 0. 014| 0. 053| 0. 008| 0. 002| Adjusted R^2| 0. 780| 0. 7| 0. 517| 0. 815| F-test| 0. 010*| 0. 01*| 0. 0051*| 0. 077**| Surya Nepal Pvt. Ltd Table 7 Independent Variables| Model-1| Model-2| Model-3| Model-4| ARCP| -0. 003|   |   |   | ACIP|   | 0. 907|   |   | APP|   |   | -0. 008|   | CCC|   |   |   | 0. 953| CR| 0. 275| 0. 360| 0. 221| 0. 275 | FL| -1. 188| -1. 599| -0. 055| -1. 188| Lns| 0. 245| 0. 224| 0. 069| 0. 245| Adjusted R^2| 0. 770| 0. 67| 0. 71| 0. 79| F-test| 0. 0876**| 0. 0035*| 0. 012*| 0. 0144*| In the above table, F-test has been done at 5%=* and 10% =**significance of level. Table 6 and 7 presents the regression result of two firms of 4 models.According to the table 6 and 7, ARCP and ROA have negatively correlated. For the both Company if there is 1% increase in the days of collection period than less than 1% will decrease on the value of profitability. Similarly, for the both firm, liquidity ratio and size of firm is positively correlated. It means, increases in the sales that will increase the profit volume. Positive value of CR and LNS but negative value of financial leverage (FL) is accepted by all models. This means, if loan amount is increased by 1% then profit will decreased by 0. 21% in case of Dabur but more than 11% in case of Surya Nepal.All the, result revealed that, to increase the profit, fi rm should decrease the loan amount. According to the Model 4 from table 6, there is negative relationship between ROA and cash conversion cycle. This means, to increase the profit, Dabur should reduce the CCC. It is also supported by the theory of higher the working capital leads to the lower of profitability. On the other hand, model 4 from table 7 reveals that there is positive relationship between ROA and CCC. This result is beyond the theory and if Surya Nepal wants to increase its profit then it should increase the Working capital.It may the cause of poor situation of Nepalese economy, nature of business as well as less concentration on environment management that is leading to keep higher amount of stock. According to table 6, model 1 , 2 ,3 and 4 explained the dependent variable by independent in the portion of 78% , 77% , 51%, and 81 % respectively and remaining portion is due to other element. But in the table 7, dependent variable (ROA) is explained by independent in the f orm of 77%, 67%, 71%, and 79% and remaining part is covered by other elements. CHAPTER-V CONCLUSION AND RECOMMENDATION 5. Conclusion and RecommendationWorking capital management is the same of liquidity management and its related inversely with profitability but this theory always doesn't work. Here, I have found the different relationship between the component of working capital and profitability by taking financial data of Dabur Nepal Ltd and Surya Nepal Pvt. Ltd manufacturing from 2006-2011. This study appears that there is a negative significance relationship between average receivables collection period (ARCP), average conversion inventory period (ACIP, only case for Dabur), average payment period (APP) and the profitability measures.It is proved also a negative relationship between the cash conversion cycle (CCC) from the data of Dabur Company but it is not the result of universal fact because it is also proved that there can be a positive relationship between CCC and Profitab ility. The reason behind this can be the political risk, poor economy, lack of availability of raw materials and delivery of goods and services in time due to the labor union problem, increasing in supplier power, unavailability of credit facility, poor management of current assets and lack of efficient procedure and subsidy facility from government.After this analysis, the study recommends for the firms to manage their working capital efficiently to achieve the optimal profitability. Thus, the firms can manage their working capital through reduce the length time between sell the goods and receive cash of sales, it can do that by accelerating its collections. And it also reduce the length time between convert the raw materials into finished goods to sell these goods through. On the other hand the firms should shorten the length time between purchase goods to pay their purchases.All these will lead to shorten the cash conversion cycle and then lead to achieve the optimal profitabilit y. Moreover, we can't say that there will be lower profit due to higher CCC because due to the environmental factors the component of working capital can be influenced and result can go beyond the planning and objective. In the context of Nepal, where the practices of working capital management is poor and as a result firms are generating lower profit. Secondly, long procedure of raw material conversion and delay of payment also reduces the profit of firm.It can be the one cause of Positive relationship between CCC and profitability in case of Surya Nepal Pvt. Ltd. Therefore, reduction of working capital is not only best solution because environment analysis is also important factor. References: Amit, K. Malik, Debashish Sur and Debdas Rakshit (2005). Working Capital and Profitability: A Study on their Relationship with Reference to Selected Companies in Indian Pharmaceutical Industry, GITAM Journal of Management. 3: 51-62. 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